Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Example of compound coenzyme (coenzyme A) for injection Mobile phase composition: 0.05mol/L phosphate buffer Column brand: SinoPak C18 chromatographic column 4.6×150 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV210nm Injection volume: 10 μL

Analysis example of Yanhuning for injection Mobile phase composition: acetonitrile/0.1% glacial acetic acid=50/50 Column brand: SinoPakPhenyl 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV250nm Injection volume: 10 μL

Analysis example of Tyvanin tartrate Mobile phase composition: A: 0.01mol/L ammonium acetate (pH adjusted to 3.0 by glacial acetic acid); B: acetonitrile Column brand: SinoChrom ODS-BP 4.6×250 mm Flow rate/flow: 1.0 mL/min Detection wavelength: UV254...