Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Mobile phase: methanol: 0.3% trifluoroacetic acid aqueous solution=45:55(V:V) Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Wavelength: 230 nm Injection volume: 10 µL Temperature: 30 ℃

Mobile phase: methanol: 0.5% glacial acetic acid aqueous solution = 70:30 (V: V) Chromatographic column: Supersil ODS2 5μm4.6×200mm Flow rate: 1.0mL/min Detection wavelength: 315nm Injection volume: 20µL Column temperature: 30℃