Mobile phase: mobile phase A: methanol, mobile phase B: 10 mmol/L pH=7 phosphate buffer Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: Ex473 nm, Em535 nm Injection volume: 10 µL Column temperature: 35 ℃ 1. p-phenylenediamine 2...

Mobile phase: Mobile phase A: Acetonitrile Mobile phase B: 20mmol/L ammonium acetate solution Column: SinoChrom ODS-BP 5µm 4.6×150mm Flow rate: 1.0mL/min Detection: Ex473nm, Em535nm Injection volume: 10µL Column temperature: 35℃ 1- putrescine; 2-cada...

Mobile phase: A: acetonitrile; B: water Column: Supersil ODS2 5 µm4.6×250 mm Flow rate: 1.0 mL/min Detection: 220 nm Injection volume: 10 µL Column temperature: 30 ℃

Mobile phase: methanol/water=60/40 Column: Hypersil ODS2 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: 234 nm Injection volume: 2 µL Column temperature: 30 ℃

Mobile phase: methanol: water=50:50 Column: SinoChrom ODS-BP 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection wavelength: 235 nm Injection volume: 10 µL Column temperature: 30 ℃ 1- methomyl oxime

Mobile phase: acetonitrile: 0.1% formic acid = 2:98 Column: SinoChrom ODS-BP 5 µm 4.6×250 mm Flow rate: 1.0 mL/min Detection: RI Injection volume: 10 µL Column temperature: 30 ℃ 1-flufenuron